Utilising retention correlation for the separation of oligostyrenes by coupled-column liquid chromatography

被引：17

作者：

Gray, MJ ^{[1
]}

Dennis, GR ^{[1
]}

Slonecker, PJ ^{[1
]}

Shalliker, RA ^{[1
]}

机构：

[1] Univ Western Sydney, Nanoscale Org & Dynam Grp, Parramatta, NSW 1797, Australia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1073卷 / 1-2期

关键词：

n-butyloligostyrenes; liquid chromatography; C-18; column;

D O I：

10.1016/j.chroma.2004.10.071

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of it = 2-5 n-butyloligostyrenes has been illustrated by reversed-phase reversed-phase (RP-RP) coupled-column liquid chromatography. The coupled-column separation has been achieved by use of a C-18 column with methanol as the mobile phase followed by a DiamondBond C-18 column with acetonitrile (ACN) mobile phase. The DiamondBond C-18 is a hybrid carbon clad zirconia (CCZ)-C-18 stationary phase. Unlike a C-18-carbon clad zirconia two-dimensional liquid chromatographic system, which is orthogonal, the C-18 and DiamondBond C-18 columns combination exhibit correlations based upon the molecular weight of n-butyloligostyrenes. Using an alternative strategy to two-dimensional liquid chromatography, the molecular weight dependence displayed by both the C18 column and DiamondBond C-18 has been used to increase throughput or decrease analysis time in the analysis of the n-butyloligostyrenes. However, this is at the expense of a portion of the two-dimensional peak capacity displayed by the C-18-carbon clad zirconia system. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：3 / 9

页数：7

共 21 条

[1] MEASUREMENT OF CYCLOPENTENYL CYTOSINE 5'-TRIPHOSPHATE IN-VITRO AND IN-VIVO BY MULTIDIMENSIONAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

AGBARIA, R ;

FORD, H ;

KELLEY, JA ;

XIE, FM ;

POLITI, P ;

GREM, JL ;

COONEY, DA ;

MARQUEZ, VE ;

ALLEGRA, CJ ;

JOHNS, DG .

ANALYTICAL BIOCHEMISTRY, 1993, 213 (01) :90-96

[2] AUTOMATED INSTRUMENTATION FOR COMPREHENSIVE 2-DIMENSIONAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PROTEINS [J].

BUSHEY, MM ;

JORGENSON, JW .

ANALYTICAL CHEMISTRY, 1990, 62 (02) :161-167

[3] 2-DIMENSIONAL COLUMN LIQUID-CHROMATOGRAPHIC TECHNIQUE FOR RESOLUTION OF COMPLEX-MIXTURES [J].

ERNI, F ;

FREI, RW .

JOURNAL OF CHROMATOGRAPHY, 1978, 149 (FEB) :561-569

[4] SAMPLE DIMENSIONALITY - A PREDICTOR OF ORDER-DISORDER IN COMPONENT PEAK DISTRIBUTION IN MULTIDIMENSIONAL SEPARATION [J].

GIDDINGS, JC .

JOURNAL OF CHROMATOGRAPHY A, 1995, 703 (1-2) :3-15

[5] 2-DIMENSIONAL ANALYSIS OF COPOLYMERS BY SIZE-EXCLUSION CHROMATOGRAPHY AND GRADIENT-ELUTION REVERSED-PHASE PRECIPITATION CHROMATOGRAPHY [J].

GLOCKNER, G ;

VANDENBERG, JHM ;

MEIJERINK, NLJ ;

SCHOLTE, TG ;

KONINGSVELD, R .

JOURNAL OF CHROMATOGRAPHY, 1984, 317 (DEC) :615-624

[6] Two dimensional reversed-phase-reversed-phase separations -: Isomeric separations incorporating C18 and carbon clad zirconia stationary phases [J].

Gray, M ;

Dennis, GR ;

Wormell, P ;

Shalliker, RA ;

Slonecker, P .

JOURNAL OF CHROMATOGRAPHY A, 2002, 975 (02) :285-297

[7] Comprehensive two-dimensional separations of complex mixtures using reversed-phase reversed-phase liquid chromatography [J].

Gray, MJ ;

Dennis, GR ;

Slonecker, PJ ;

Shalliker, RA .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1041 (1-2) :101-110

[8] Separation of oligostyrene isomers in a complex mixture using two-dimensional heart-cutting reversed-phased liquid chromatography [J].

Gray, MJ ;

Dennis, GR ;

Slonecker, PJ ;

Shalliker, RA .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1028 (02) :247-257

[9] Evaluation of the two-dimensional reversed-phase-reversed-phase separations of low-molecular mass polystyrenes [J].

Gray, MJ ;

Dennis, GR ;

Slonecker, PJ ;

Shalliker, RA .

JOURNAL OF CHROMATOGRAPHY A, 2003, 1015 (1-2) :89-98

[10] Comprehensive coupled reversed-phase reversed-phase separations of a complex isomeric mixture [J].

Gray, MJ ;

Sweeney, AP ;

Dennis, GR ;

Slonecker, PJ ;

Shalliker, RA .

ANALYST, 2003, 128 (06) :598-604

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