The chemical-in-μwell: a high-throughput technique for identifying solutes eliciting a chemotactic response in motile bacteria

被引：12

作者：

Armitano, Joshua ^{[1
,2
]}

Baraquet, Claudine ^{[1
]}

Michotey, Valerie ^{[3
]}

Mejean, Vincent ^{[1
,2
]}

Jourlin-Castelli, Cecile ^{[1
,2
]}

机构：

[1] CNRS, UPR 9043, Inst Microbiol Mediterranee, Chim Bacterienne Lab, F-13402 Marseille 20, France

[2] Univ Aix Marseille, Marseille, France

[3] Univ Aix Marseille 2, Ctr Oceanol Marseille, CNRS, Lab Microbiol Geochim & Ecol Marine,UMR 6117, F-13288 Marseille 9, France

来源：

RESEARCH IN MICROBIOLOGY | 2011年 / 162卷 / 09期

关键词：

Marine bacteria; Bacterial motility; Chemotaxis; Attractant; Repellent; Phenotype microarrays; SHEWANELLA-ONEIDENSIS MR-1; ELECTRON-ACCEPTORS; ENERGY TAXIS; CHEMORECEPTORS; GROWTH; OXIDE;

D O I：

10.1016/j.resmic.2011.03.001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacteria, and in particular marine bacteria, can be found in environments that are poor in nutrients. To survive, they are able to move toward more favorable niches by a mechanism called chemotaxis, whose first step consists in the detection of substrates by chemoreceptors. We developed a chemotactic assay enabling rapid testing of several hundred different solutes and we identified several molecules eliciting a chemotactic response from two aquatic Shewanella species. We propose that this assay be used for other bacteria to determine the repertoire of chemotactic molecules, generally not clearly elucidated. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

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收藏

页码：934 / 938

页数：5

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