Preparation of functional recombinant protein from E-coli using a nondetergent sulfobetaine

被引：6

作者：

Chong, YC

Chen, HW ^{[1
]}

机构：

[1] Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan

[2] Natl Taiwan Univ, Taipei 10764, Taiwan

来源：

BIOTECHNIQUES | 2000年 / 29卷 / 06期

关键词：

D O I：

10.2144/00296bm01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：1166 / 1167

页数：2

共 8 条

[1]

Ausubel F.M., 1992, CURRENT PROTOCOLS MO

[2] Expression and characterization of the recombinant catalytic subunit of casein kinase II from the yeast Yarrowia lipolytica in Escherichia coli [J].

Benetti, PH ;

Kim, SI ;

Chaillot, D ;

Canonge, M ;

Chardot, T ;

Meunier, JC .

PROTEIN EXPRESSION AND PURIFICATION, 1998, 13 (03) :283-290

[3]

CHEN H, 1996, DNA CELL BIOL, V14, P681

[4] SOLUBILIZATION AND PURIFICATION OF ENZYMATICALLY ACTIVE GLUTATHIONE-S-TRANSFERASE (PGEX) FUSION PROTEINS [J].

FRANGIONI, JV ;

NEEL, BG .

ANALYTICAL BIOCHEMISTRY, 1993, 210 (01) :179-187

[5] THE P50 SUBUNIT OF NF-KAPPA-B ASSOCIATES WITH THE NF-IL6 TRANSCRIPTION FACTOR [J].

LECLAIR, KP ;

BLANAR, MA ;

SHARP, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (17) :8145-8149

[6] Functional properties of the separate subunits of human DNA helicase II Ku autoantigen [J].

Ochem, AE ;

Skopac, D ;

Costa, M ;

Rabilloud, T ;

Vuillard, L ;

Simoncsits, A ;

Giacca, M ;

Falaschi, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (47) :29919-29926

[7] HALOPHILIC PROTEIN STABILIZATION BY THE MILD SOLUBILIZING AGENTS NONDETERGENT SULFOBETAINES [J].

VUILLARD, L ;

MADERN, D ;

FRANZETTI, B ;

RABILLOUD, T .

ANALYTICAL BIOCHEMISTRY, 1995, 230 (02) :290-294

[8] NON-DETERGENT SULFOBETAINES - A NEW CLASS OF MILD SOLUBILIZATION AGENTS FOR PROTEIN-PURIFICATION [J].

VUILLARD, L ;

BRAUNBRETON, C ;

RABILLOUD, T .

BIOCHEMICAL JOURNAL, 1995, 305 :337-343

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