Structural characterization of the exopolysaccharide produced by Lactobacillus acidophilus LMG9433

被引:46
作者
Robijn, GW
Gallego, RG
vandenBerg, DJC
Haas, H
Kamerling, JP
Vliegenthart, JFG
机构
[1] UNIV UTRECHT,BIJVOET CTR,DEPT BIOORGAN CHEM,NL-3508 TB UTRECHT,NETHERLANDS
[2] UNILEVER RES LABS VLAARDINGEN,NL-3130 AC VLAARDINGEN,NETHERLANDS
关键词
lactic acid bacteria; Lb-acidophilus; exopolysaccharide structure;
D O I
10.1016/0008-6215(96)00097-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The exopolysaccharide produced by Lactobacillus acidophilus LMG9433 in a semi-defined medium was found to be a charged heteropolymer, with a composition of D-glucose, D-galactose, D-glucuronic acid, and 2-acetamido-2-deoxy-D-glucose in molar ratios of 2:1:1:1. By means of methylation analysis, uronic acid degradation, de-N-acetylation/deamination, partial acid hydrolysis, and 1D/2D NMR studies the polysaccharide was demonstrated to consist of repeating units with the following structure: [GRAPHICS] (C) 1996 Elsevier Science Ltd.
引用
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页码:203 / 218
页数:16
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