Is lysozyme really the ideal model protein?

被引：23

作者：

Chayen, NE ^{[1
]}

Saridakis, E ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Div Biomed Sci, Biol Struct & Funct Sect, London SW7 2AZ, England

来源：

JOURNAL OF CRYSTAL GROWTH | 2001年 / 232卷 / 1-4期

关键词：

biocrystallization; nucleation; surfaces; biological macromolecules; lysozyme; proteins;

D O I：

10.1016/S0022-0248(01)01203-9

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

A series of potential heterogeneous nucleants were added to metastable solutions of lysozyme and of four other model proteins, as well as to a few target proteins. Some of the substances markedly affected the nucleation of lysozyme crystals, but did not influence the other proteins. The results for other proteins were disappointing because these nucleants have not turned out to be the universal nucleants we were hoping for. However, having worked so well for lysozyme, they may yet prove useful in individual cases. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：262 / 264

页数：3

共 10 条

[1] The role of oil in macromolecular crystallization
Chayen, NE
[J]. STRUCTURE, 1997, 5 (10) : 1269 - 1274
[2] CHAYEN NE, 1993, PROTEIN SCI, V2, P113
[3] Effect of high hydrostatic pressure on nucleation and growth of protein crystals
Lorber, B
Jenner, G
Giege, R
[J]. JOURNAL OF CRYSTAL GROWTH, 1996, 158 (1-2) : 103 - 117
[4] HETEROGENEOUS AND EPITAXIAL NUCLEATION OF PROTEIN CRYSTALS ON MINERAL SURFACES
MCPHERSON, A
SHLICHTA, P
[J]. SCIENCE, 1988, 239 (4838) : 385 - 387
[5] Nanev CN, 2000, CRYST RES TECHNOL, V35, P189, DOI 10.1002/(SICI)1521-4079(200002)35:2<189::AID-CRAT189>3.0.CO
[6] 2-U
[7] Effects of a magnetic field on the nucleation and growth of protein crystals
Sazaki, G
Yoshida, E
Komatsu, H
Nakada, T
Miyashita, S
Watanabe, K
[J]. JOURNAL OF CRYSTAL GROWTH, 1997, 173 (1-2) : 231 - 234
[8] Stura E., 1999, Protein crystallization: Techniques, strategies, and tips: A laboratory manual, P141
[9] Heterogeneous nucleation (and adhesion) of lysozyme crystals
Tsekova, D
Dimitrova, S
Nanev, CN
[J]. JOURNAL OF CRYSTAL GROWTH, 1999, 196 (2-4) : 226 - 233
[10] Effect of a magnetic field gradient on the crystallization of hen lysozyme
Wakayama, NI
Ataka, M
Abe, H
[J]. JOURNAL OF CRYSTAL GROWTH, 1997, 178 (04) : 653 - 656

← 1 →