Nanoparticle immunoagglutination Rayleigh scatter assay to complement microparticle immunoagglutination Mie scatter assay in a microfluidic device

被引:19
作者
Heinze, Brian C. [1 ]
Yoon, Jeong-Yeol [1 ]
机构
[1] Univ Arizona, Dept Agr & Biosyst Engn, Tucson, AZ 85721 USA
关键词
Gold nanoparticles; Latex particles; E; coli; Lab on a chip; Particle immunoassay; ESCHERICHIA-COLI; IMMUNOSENSOR; IMMUNOASSAY; ENZYME;
D O I
10.1016/j.colsurfb.2011.02.024
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In this work, particle immunoagglutination assays for pathogen detection, utilizing light scattering measurements at a fixed angle from incident light delivery, are explored in both Rayleigh and Mie scatter regimes through scatter intensity simulations and compared to experimental results. The average size of immunoagglutinated particles obtained from microscope images correspond to the particle size parameter from simulations. Mie scatter measurements yield a greater signal increase with increasing pathogen concentration than Rayleigh scatter measurements, but with a non-monotonic relationship that is not observed in the Rayleigh scatter regime. These two similar yet distinctly different sources of information could easily be integrated into a single device through fabrication of a simple microfluidic device containing two y-channels, each for performing the respective light scattering measurement. Escherichia coli was used as a representative target, and detected in a microfluidic device down to a concentration of 1 colony forming units (CFU) per mL. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:168 / 173
页数:6
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