Structure of the acidic O-specific polysaccharide from Proteus vulgaris O39 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid

被引：16

作者：

Kondakova, AN

Perepelov, AV

Bartodziejska, B

Shashkov, AS

Senchenkova, SN

Wykrota, M

Knirel, YA

Rozalski, A

机构：

[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia

[2] Univ Lodz, Inst Microbiol & Immunol, Dept Immunobiol Bacteria, PL-90237 Lodz, Poland

来源：

CARBOHYDRATE RESEARCH | 2001年 / 333卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

Proteus vulgaris; O-specific polysaccharide; 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid; pseudaminic acid; selective cleavage; triflic acid solvolysis;

D O I：

10.1016/S0008-6215(01)00145-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The O-specific polysaccharide of Proteus vulgaris O39 was found to contain a new acidic component of Proteus lipopolysaccharides, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Pse5Ac7Ac). The following structure of the polysaccharide was determined by NMR spectroscopy, including 2D H-1,H-1 COSY, TOCSY, ROESY. and H-1, C-13 HMQC experiments, along with selective cleavage of the polysaccharide by solvolysis with anhydrous trifluoromethanesulfonic (triflic) acid: -->8)-beta -Psep 5Ac7Ac-(2 --> 3)-alpha -L-FucpNAc-(1 --> 3)-alpha -o-GlcpNAc-( 1 --> dThe structure established is unique among the O-specific polysaccharides, which is in accordance with classification of the strain studied into a separate Proteus serogroup. (C) 2001 Published by Elsevier Science Ltd.

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页码：241 / 249

页数：9

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