Cloning and functional expression of rat ether-a-go-go-like K+ channel genes

被引：43

作者：

Engeland, B ^{[1
]}

Neu, A ^{[1
]}

Ludwig, J ^{[1
]}

Roeper, J ^{[1
]}

Pongs, O ^{[1
]}

机构：

[1] Zentrum Mol Neurobiol, Inst Neurale Signalverarbeitung, ZMNH, D-20246 Hamburg, Germany

来源：

JOURNAL OF PHYSIOLOGY-LONDON | 1998年 / 513卷 / 03期

关键词：

D O I：

10.1111/j.1469-7793.1998.647ba.x

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

1. Screening of rat cortex cDNA resulted in cloning of two complete and one partial orthologue of the Drosophila ether-a-go-go-like K+ channel (elk). 2. Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed predominant expression of rat elk mRNAs in brain. Each rat elk mRNA showed a distinct, but overlapping expression pattern in different rat brain areas. 3. Transient transfection of Chinese hamster ovary (CHO) cells with rat elk1 or rat elk2 cDNA gave rise to voltage-activated K+ channels with novel properties. 4. RELK1 channels mediated slowly activating sustained potassium currents. The threshold for activation was at -90 mV. Currents were insensitive to tetraethylammonium (TEA) and 4-aminopyridine (4-AP), but were blocked by micromolar concentrations of Ba2+. RELK1 activation kinetics were not dependent on prepulse potential like REAG-mediated currents. 5. RELK2 channels produced currents with a fast inactivation component and HERG-like tail currents. RELK2 currents were not sensitive to the HERG channel blocker E4031.

引用

页码：647 / 654

页数：8

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