Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes

被引：143

作者：

Ho, YP

Kung, MC

Yang, S

Wang, TH ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Mech Engn, Baltimore, MD 21218 USA

[2] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21218 USA

[3] Johns Hopkins Sch Med, Dept Emergency Med, Baltimore, MD 21218 USA

来源：

NANO LETTERS | 2005年 / 5卷 / 09期

关键词：

D O I：

10.1021/nl050888v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate a hybridization detection method using multicolor oligonucleotide-functionalized quantum dots as nanoprobes. In the presence of various target sequences, combinatorial self-assembly of the nanoprobes via independent hybridization reactions leads to the generation of discernible sequence-specific spectral codings. Detection of single-molecule hybridization is achieved by measuring colocalization of individual nanoprobes. Genetic analysis for anthrax pathogenicity through simultaneous detection of multiple relevant sequences is demonstrated using this novel biosensing method as proof-of-concept.

引用

页码：1693 / 1697

页数：5

共 17 条

[1] Real-time multicolor DNA detection with chemoresponsive diffraction gratings and nanoparticle probes [J].