The utility of green fluorescent protein in transgenic plants

被引：151

作者：

Stewart, CN ^{[1
]}

机构：

[1] Univ N Carolina, Dept Biol, Greensboro, NC 27402 USA

来源：

PLANT CELL REPORTS | 2001年 / 20卷 / 05期

关键词：

fluorescent proteins; GFP; gene expression; marker genes; plant transformation;

D O I：

10.1007/s002990100346

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as those including the S65T chromophore mutation and those with dual excitation peaks, are discussed. The review also focuses on the utility of GFP as a visual selectable marker in aiding the plant transformation process; GFP has been more important in monocot transformation compared with dicot transformation. Finally, the potential utility of new fluorescent proteins is speculated upon.

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收藏

页码：376 / 382

页数：7

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