Phosphorylation of cold shock domain/Y-box proteins by ERK2 and GSK3β and repression of the human VEGF promoter

被引：57

作者：

Coles, LS

Lambrusco, L

Burrows, J

Hunter, J

Diamond, P

Bert, AG

Vadas, MA

Goodall, GJ

机构：

[1] Inst Med & Vet Sci, Hanson Inst, Div Human Immunol, Adelaide, SA 5000, Australia

[2] Univ Adelaide, Dept Med, Adelaide, SA 5000, Australia

来源：

FEBS LETTERS | 2005年 / 579卷 / 24期

基金：

英国医学研究理事会;

关键词：

vascular endothelial growth factor; cold shock domain protein; Y-box protein; gene repression;

D O I：

10.1016/j.febslet.2005.08.075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hypoxia responsive region (HRR) of the VEGF promoter plays a key role in regulating VEGF expression. We found that the cold shock domain (Y-box) repressor proteins, dbpA and dbpB/YB-1, bind distinct strands of the human VEGF HRR. We find both dbpA and dbpB are phosphorylated by ERK2 and GSK3 beta in vitro, and the binding of dbpB to single-strand VEGF HRR DNA is regulated by this phosphorylation. These findings suggest the ERK/MAPK and PI3K pathways may regulate VEGF expression in part through regulating the action of these repressor proteins. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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收藏

页码：5372 / 5378

页数：7

相关论文

共 36 条

[1] OS-9 interacts with hypoxia-inducible factor 1α and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1α [J].

Baek, JH ;

Mahon, PC ;

Oh, J ;

Kelly, B ;

Krishnamachary, B ;

Pearson, M ;

Chan, DA ;

Giaccia, AJ ;

Semenza, GL .

MOLECULAR CELL, 2005, 17 (04) :503-512

[2] MAP kinases and hypoxia in the control of VEGF expression [J].

Berra, E ;

Pagès, G ;

Pouysségur, J .

CANCER AND METASTASIS REVIEWS, 2000, 19 (1-2) :139-145

[3] A novel mechanism of repression of the vascular endothelial growth factor promoter, by single strand DNA binding cold shock domain (Y-box) proteins in normoxic fibroblasts [J].

Coles, LS ;

Diamond, P ;

Lambrusco, L ;

Hunter, J ;

Burrows, J ;

Vadas, MA ;

Goodall, GJ .

NUCLEIC ACIDS RESEARCH, 2002, 30 (22) :4845-4854

[4] An ordered array of cold shock domain repressor elements across tumor necrosis factor-responsive elements of the granulocyte-macrophage colony-stimulating factor promoter [J].

Coles, LS ;

Diamond, P ;

Occhiodoro, F ;

Vadas, MA ;

Shannon, MF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (19) :14482-14493

[5] Crystal structure of glycogen synthase kinase 3β:: Structural basis for phosphate-primed substrate specificity and autoinhibition [J].

Dajani, R ;

Fraser, E ;

Roe, SM ;

Young, N ;

Good, V ;

Dale, TC ;

Pearl, LH .

CELL, 2001, 105 (06) :721-732

[6]

DAVIS RJ, 1993, J BIOL CHEM, V268, P14553

[7] Cold shock domain factors activate the granulocyte-macrophage colony-stimulating factor promoter in stimulated Jurkat T cells [J].

Diamond, P ;

Shannon, MF ;

Vadas, MA ;

Coles, LS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (11) :7943-7951

[8] A common phosphate binding site explains the unique substrate specificity of GSK3 and its inactivation by phosphorylation [J].

Frame, S ;

Cohen, P ;

Biondi, RM .

MOLECULAR CELL, 2001, 7 (06) :1321-1327

[9] A superfamily of proteins that contain the cold-shock domain [J].

Graumann, PL ;

Marahiel, MA .

TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (08) :286-290

[10] Regulation of GSK-3: A cellular multiprocessor [J].

Harwood, AJ .

CELL, 2001, 105 (07) :821-824

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