Detection of icaA and icaD loci by polymerase chain reaction and biofilm formation by Staphylococcus epidermidis isolated from dialysate and needles in a dialysis unit

被引:52
作者
Chaieb, K
Mahdouani, K
Bakhrouf, A
机构
[1] Fac Pharm, Lab Anal & Controle Polluants Chim & Microbiol En, Monastir 5000, Tunisia
[2] Hop Ibn El Jazzar, Bacteriol Lab, Kairouan 3140, Tunisia
关键词
medical devices; S; epidermidis; Biofilm; ica operon;
D O I
10.1016/j.jhin.2005.05.014
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Staphylococcus epidermidis, a coagulase-negative staphylococcus, is a major cause of infections associated with indwelling medical, devices. Certain strains produce slime and form biofilm on polymer surfaces, where their pathogenicity is associated with biofilm formation. In this report, we investigated the presence or absence of the intercellular adhesion icaA and icaD genes by polymerase chain reaction, and phenotypic biofilm production was examined by qualitative Congo red agar (CRA) assay. A total of 32 strains of S. epidermidis were identified from dialysates and needles 4 h after the initiation of dialysis. Qualitative biofilm production revealed that 16 (50%) strains produced slime on CRA plates. Among the 23 strains positive for the ica operon, 15 were biofilm positive on CRA, eight were biofilm negative, and one was icaA and icaD) negative but produced slime. These results show that the ability of S. epidermidis to produce slime is not associated with the presence of icaA and icaD genes. (c) 2005 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:225 / 230
页数:6
相关论文
共 36 条
[1]   Search for the insertion element IS256 within the ica locus of Staphylococcus epidermidis clinical isolates collected from biomaterial-associated infections [J].
Arciola, CR ;
Campoccia, D ;
Gamberini, S ;
Rizzi, S ;
Donati, ME ;
Baldassarri, L ;
Montanaro, L .
BIOMATERIALS, 2004, 25 (18) :4117-4125
[2]   Detection of slime production by means of an optimised Congo red agar plate test based on a colourimetric scale in Staphylococcus epidermidis clinical isolates genotyped for ica locus [J].
Arciola, CR ;
Campoccia, D ;
Gamberini, S ;
Cervellati, M ;
Donati, E ;
Montanaro, L .
BIOMATERIALS, 2002, 23 (21) :4233-4239
[3]   Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheter-associated infections [J].
Arciola, CR ;
Baldassarri, L ;
Montanaro, L .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (06) :2151-2156
[4]  
Arciola CR, 1998, J BIOMED MATER RES, V42, P1
[5]   A rapid PCR method for the detection of slime-producing strains of Staphylococcus epidermidis and S-aureus in periprosthesis infections [J].
Arciola, CR ;
Collamati, S ;
Donati, E ;
Montanaro, L .
DIAGNOSTIC MOLECULAR PATHOLOGY, 2001, 10 (02) :130-137
[6]   Effect of iron limitation on slime production by Staphylococcus aureus [J].
Baldassarri, L ;
Bertuccini, L ;
Ammendolia, MG ;
Arciola, CR ;
Montanaro, L .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2001, 20 (05) :343-345
[7]  
BAYSTON R, 1972, DEV MED CHILD NEUROL, V14, P25
[8]   MICROBIAL BIOFILMS [J].
COSTERTON, JW ;
LEWANDOWSKI, Z ;
CALDWELL, DE ;
KORBER, DR ;
LAPPINSCOTT, HM .
ANNUAL REVIEW OF MICROBIOLOGY, 1995, 49 :711-745
[9]   Anaerobic conditions induce expression of polysaccharide intercellular adhesin in Staphylococcus aureus and Staphylococcus epidermidis [J].
Cramton, SE ;
Ulrich, M ;
Götz, F ;
Döring, G .
INFECTION AND IMMUNITY, 2001, 69 (06) :4079-4085
[10]   Virulence of Staphylococcus epidermidis in a mouse model: Significance of extracellular slime [J].
Deighton, MA ;
Borland, R ;
Capstick, JA .
EPIDEMIOLOGY AND INFECTION, 1996, 117 (02) :267-280