Characterization of Langat virus antigenic determinants defined by monoclonal antibodies to E,NS1 and preM and identification of a protective, non-neutralizing preM-specific monoclonal antibody

被引:37
作者
IaconoConnors, LC
Smith, JF
Ksiazek, TG
Kelley, CL
Schmaljohn, CS
机构
[1] USA,MED RES INST INFECT DIS,DIV VIROL,FREDERICK,MD 21702
[2] USA,MED RES INST INFECT DIS,DIV DIS ASSESSMENT,FT DETRICK,MD 21702
关键词
hybridomas; monoclonal antibodies; tick-borne encephalitis;
D O I
10.1016/0168-1702(96)01325-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hybridomas secreting monoclonal antibodies (MAb) to the tick-borne encephalitis (TEE) group virus, Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterization. Of these MAb, 14 were specific for LGTV E glycoprotein, two for the NS1 protein, and three for preM protein. The two NS1-specific MAb and two of the E-specific MAb reacted with all six of the other TEE group viruses tested while the remainder of the E-specific MAb failed to recognize at least one of the viruses. None of the MAb neutralized LGTV in cell culture assays, but one of the preM-specific MAb protected weanling mice against a virulent LGTV challenge. Although protective antibodies to E and NS1 proteins of TEE viruses were reported, our data provided the first evidence for protection by a non-neutralizing antibody to the preM or M protein of any of the tick-borne flaviviruses.
引用
收藏
页码:125 / 136
页数:12
相关论文
共 40 条
[1]  
[Anonymous], 1990, VIROLOGY
[2]   CHARACTERIZATION OF HANTAAN VIRUS ENVELOPE GLYCOPROTEIN ANTIGENIC DETERMINANTS DEFINED BY MONOCLONAL-ANTIBODIES [J].
ARIKAWA, J ;
SCHMALJOHN, AL ;
DALRYMPLE, JM ;
SCHMALJOHN, CS .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :615-624
[3]   DENGUE VIRUS PREMEMBRANE AND MEMBRANE-PROTEINS ELICIT A PROTECTIVE IMMUNE-RESPONSE [J].
BRAY, M ;
LAI, CJ .
VIROLOGY, 1991, 185 (01) :505-508
[4]   PARAMETERS AFFECTING ASCITES TUMOR-FORMATION IN MICE AND MONOCLONAL-ANTIBODY PRODUCTION [J].
BRODEUR, BR ;
TSANG, P ;
LAROSE, Y .
JOURNAL OF IMMUNOLOGICAL METHODS, 1984, 71 (02) :265-272
[5]   NEUTRALIZATION OF YELLOW-FEVER VIRUS STUDIED USING MONOCLONAL AND POLYCLONAL ANTIBODIES [J].
BUCKLEY, A ;
GOULD, EA .
JOURNAL OF GENERAL VIROLOGY, 1985, 66 :2523-2531
[6]   ANTIGENIC RELATIONSHIPS BETWEEN FLAVIVIRUSES AS DETERMINED BY CROSS-NEUTRALIZATION TESTS WITH POLYCLONAL ANTISERA [J].
CALISHER, CH ;
KARABATSOS, N ;
DALRYMPLE, JM ;
SHOPE, RE ;
PORTERFIELD, JS ;
WESTAWAY, EG ;
BRANDT, WE .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :37-43
[7]   REDUCTION OF YELLOW-FEVER VIRUS MOUSE NEUROVIRULENCE BY IMMUNIZATION WITH A BACTERIALLY SYNTHESIZED NON-STRUCTURAL PROTEIN (NS1) FRAGMENT [J].
CANE, PA ;
GOULD, EA .
JOURNAL OF GENERAL VIROLOGY, 1988, 69 :1241-1246
[8]   FLAVIVIRUS GENOME ORGANIZATION, EXPRESSION, AND REPLICATION [J].
CHAMBERS, TJ ;
HAHN, CS ;
GALLER, R ;
RICE, CM .
ANNUAL REVIEW OF MICROBIOLOGY, 1990, 44 :649-688
[9]   SEROLOGICAL RELATIONSHIPS AMONG VIRUSES IN THE HANTAVIRUS GENUS, FAMILY BUNYAVIRIDAE [J].
CHU, YK ;
ROSSI, C ;
LEDUC, JW ;
LEE, HW ;
SCHMALJOHN, CS ;
DALRYMPLE, JM .
VIROLOGY, 1994, 198 (01) :196-204
[10]   FLAVIVIRUSES (GROUP-B ARBOVIRUSES) - CROSS-NEUTRALIZATION STUDY [J].
DEMADRID, AT ;
PORTERFIELD, JS .
JOURNAL OF GENERAL VIROLOGY, 1974, 23 (APR) :91-96