Characterization of Langat virus antigenic determinants defined by monoclonal antibodies to E,NS1 and preM and identification of a protective, non-neutralizing preM-specific monoclonal antibody

Hybridomas secreting monoclonal antibodies (MAb) to the tick-borne encephalitis (TEE) group virus, Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterization. Of these MAb, 14 were specific for LGTV E glycoprotein, two for the NS1 protein, and three for preM protein. The two NS1-specific MAb and two of the E-specific MAb reacted with all six of the other TEE group viruses tested while the remainder of the E-specific MAb failed to recognize at least one of the viruses. None of the MAb neutralized LGTV in cell culture assays, but one of the preM-specific MAb protected weanling mice against a virulent LGTV challenge. Although protective antibodies to E and NS1 proteins of TEE viruses were reported, our data provided the first evidence for protection by a non-neutralizing antibody to the preM or M protein of any of the tick-borne flaviviruses.