Prevalence of nucleic acid sequences specific for human parvoviruses, hepatitis A and hepatitis E viruses in coagulation factor concentrates

被引：39

作者：

Modrow, S. ^{[1
]}

Wenzel, J. J. ^{[1
]}

Schimanski, S. ^{[1
]}

Schwarzbeck, J. ^{[2
]}

Rothe, U. ^{[2
]}

Oldenburg, J. ^{[3
]}

Jilg, W. ^{[1
]}

Eis-Huebinger, A. M. ^{[4
]}

机构：

[1] Univ Regensburg, Inst Med Microbiol & Hyg, D-93053 Regensburg, Germany

[2] Pharm Univ Hosp Regensburg, Regensburg, Germany

[3] Univ Bonn, Med Ctr, Inst Expt Haematol & Transfus Med, D-5300 Bonn, Germany

[4] Univ Bonn, Med Ctr, Inst Virol, D-5300 Bonn, Germany

来源：

VOX SANGUINIS | 2011年 / 100卷 / 04期

关键词：

hepatitis virus; human bocavirus; iatrogenic transmission; parvovirus B19; plasma-derived coagulation factor products; recombinant coagulation factor products; B19; TRANSMISSION; BLOOD-DONORS; TRANSFUSION; INFECTION; PLASMA; PARV4; DNA; HEMOPHILIACS; PRODUCTS; EXPOSURE;

D O I：

10.1111/j.1423-0410.2010.01445.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background and Objectives Due to their high resistance to inactivation procedures, nonenveloped viruses such as parvovirus B19, human bocavirus (HBoV), human parvovirus 4 (PARV4), hepatitis A (HAV) and hepatitis E virus (HEV) pose a particular threat to blood products. Virus transmission to patients treated with blood products presents an additional burden to disease. We determined the frequency and the amount of nucleic acid specific for nonenveloped viruses in recently manufactured preparations of commercial coagulation factor concentrates. Materials and Methods At least three different batches of each of 13 different plasma-derived and recombinant coagulation factor products were tested for the presence and the amount of nucleic acid for parvovirus B19, HBoV, human parvovirus 4, hepatitis A virus and HEV by using quantitative polymerase chain reaction. Results Whereas none of the recombinant products tested positive for any of these viruses, parvovirus B19 DNA with amounts ranging between 2 x 101 and 1.3 x 103 genome equivalents/ml was detected in five plasma-derived products. In addition to parvovirus B19 genotype 1, genotypes 2 and 3 were observed in two batches of a factor VIII/von-Willebrand factor product. In two products (one factor VIII concentrate and one activated prothrombin complex concentrate), a combination of both genotypes 1 and 2 of parvovirus B19 was detected. Conclusion The data show that nucleic acids from several relevant nonenveloped viruses are not found at detectable levels in coagulation factor concentrates. In some cases, parvovirus B19 DNA was detectable at low levels. Testing of the plasma pools for the full range of parvovirus genotypes is advocated for ensuring product safety.

引用

页码：351 / 358

页数：8

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