Sex-Specific Dynamics of Global Chromatin Changes in Fetal Mouse Germ Cells

被引：31

作者：

Abe, Masanobu ^{[1
,2
]}

Tsai, Shirley Y. ^{[1
,2
]}

Jin, Seung-Gi ^{[2
,3
]}

Pfeifer, Gerd P. ^{[2
,3
]}

Szabo, Piroska E. ^{[1
,2
]}

机构：

[1] City Hope Natl Med Ctr, Dept Mol & Cellular Biol, Duarte, CA 91010 USA

[2] City Hope Natl Med Ctr, Beckman Res Inst, Duarte, CA 91010 USA

[3] City Hope Natl Med Ctr, Dept Canc Biol, Duarte, CA 91010 USA

来源：

PLOS ONE | 2011年 / 6卷 / 08期

基金：

美国国家卫生研究院;

关键词：

DE-NOVO METHYLATION; DNA METHYLATION; IMPRINTED GENES; MAMMALIAN DEVELOPMENT; OOCYTE GROWTH; MICE; EXPRESSION; HETEROCHROMATIN; GENOME; ESTABLISHMENT;

D O I：

10.1371/journal.pone.0023848

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mammalian germ cells undergo global reprogramming of DNA methylation during their development. Global DNA demethylation occurs around the time when the primordial germ cells colonize the embryonic gonads and this coincides with dynamic changes in chromatin composition. Global de novo DNA methylation takes place with remarkably different dynamics between the two sexes, prospermatogonia attaining methylation during fetal stages and oocytes attaining methylation postnatally. Our hypothesis was that dynamic changes in chromatin composition may precede or accompany the wave of global DNA de novo methylation as well. We used immunocytochemistry to measure global DNA methylation and chromatin components in male and female mouse fetal germ cells compared to control somatic cells of the gonad. We found that global DNA methylation levels sharply increased in male germ cells at 17.5 days post coitum, but remained low in female germ cells at all fetal stages. Global changes in chromatin composition: i, preceded global DNA methylation in fetal germ cells; ii, sex specifically occurred in male but not in female germ cells; iii, affected active and repressive histone marks and iv, included histone tail and histone globular domain modifications. Our data suggest that dynamic changes of chromatin composition may provide a framework for the pattern of male-specific de novo DNA methylation in prospermatogonia.

引用

页数：12

共 40 条

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