Molecular genetic basis of ribotyping

被引：43

作者：

Bouchet, Valerie ^{[1
,2
]}

Huot, Heather ^{[1
]}

Goldstein, Richard ^{[1
]}

机构：

[1] Boston Univ, Med Ctr, Sch Med, Mol Genet Sect,Div Pediat Infect Dis,Maxwell Finl, Boston, MA 02118 USA

[2] Boston Univ, Sch Publ Hlth, Dept Environm Hlth, Boston, MA 02118 USA

来源：

CLINICAL MICROBIOLOGY REVIEWS | 2008年 / 21卷 / 02期

关键词：

D O I：

10.1128/CMR.00026-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Nearly 2, 000 ribotyping-based studies exist, ranging from epidemiology to phylogeny and taxonomy. None precisely reveals the molecular genetic basis, with many incorrectly attributing detected polymorphisms to rRNA gene sequences. Based on in silico genomics, we demonstrate that ribotype polymorphisms result from sequence variability in neutral housekeeping genes flanking rRNA operons, with rRNA gene sequences serving solely as conserved, flank-linked tags. We also reveal that from such an informatics perspective, it is readily feasible a priori to design an interpretable ribotyping scheme for a genomically sequenced microbial species, and we discuss limitations to the basic restriction fragment length polymorphism-based method as well as alternate PCR ribotyping-based schemes.

引用

页码：262 / +

页数：13