The use of a fluorescent bacteriophage assay for detection of Escherichia coli O157:H7 in inoculated ground beef and raw milk

被引：75

作者：

Goodridge, L

Chen, JR

Griffiths, M ^{[1
]}

机构：

[1] Univ Guelph, Dept Food Sci, Guelph, ON N1G 2W1, Canada

[2] Univ Georgia, Ctr Food Safety & Qual Enhancement, Griffin, GA 30223 USA

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1999年 / 47卷 / 1-2期

关键词：

fluorescent; bacteriophage; detection; Escherichia coli O157 : H7; ground beef; raw milk;

D O I：

10.1016/S0168-1605(99)00010-0

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The objective of this study was to develop a fluorescent bacteriophage assay (FBA) for the detection of E. coli O157:H7 in ground beef and raw milk. The FBA is a two step assay that combines immunomagnetic separation, to separate the target organism from mixed culture, with a highly specific fluorescently stained bacteriophage to label the E. coli O157:H7 cells. When used in conjunction with flow cytometry, the FBA was able to detect 2.2 CFU/g of artificially contaminated ground beef following a 6 h enrichment. Between 10(1) and 10(2) CFU/ml of artificially contaminated raw milk were detectable after a 10 h enrichment step. The results show that the FBA is potentially useful as a rapid technique for the preliminary detection of E. coli O157:H7 in food. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：43 / 50

页数：8

相关论文

共 29 条

[21] ANTIGENIC RELATIONSHIPS OF THE LIPOPOLYSACCHARIDES OF ESCHERICHIA-HERMANNII STRAINS WITH THOSE OF ESCHERICHIA-COLI O157-H7, BRUCELLA-MELITENSIS, AND BRUCELLA-ABORTUS [J].

PERRY, MB ;

BUNDLE, DR .

INFECTION AND IMMUNITY, 1990, 58 (05) :1391-1395

[22] COLLABORATIVE TRIAL OF THE DIRECT EPIFLUORESCENT FILTER TECHNIQUE (DEFT), A RAPID METHOD FOR COUNTING BACTERIA IN MILK [J].

PETTIPHER, GL ;

FULFORD, RJ ;

MABBITT, LA .

JOURNAL OF APPLIED BACTERIOLOGY, 1983, 54 (02) :177-182

[23] RAPID ENUMERATION OF MICROORGANISMS IN FOODS BY THE DIRECT EPIFLUORESCENT FILTER TECHNIQUE [J].

PETTIPHER, GL ;

RODRIGUES, UM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 44 (04) :809-813

[24] ISOLATION AND CHARACTERIZATION OF A COLIPHAGE SPECIFIC FOR ESCHERICHIA-COLI-O157-H7 [J].

RONNER, AB ;

CLIVER, DO .

JOURNAL OF FOOD PROTECTION, 1990, 53 (11) :944-947

[25] A new route of transmission for Escherichia coli: Infection from dry fermented salami [J].

Tilden, J ;

Young, W ;

McNamara, AM ;

Custer, C ;

Boesel, B ;

LambertFair, M ;

Majkowski, J ;

Vugia, D ;

Werner, SB ;

Hollingsworth, J ;

Morris, JG .

AMERICAN JOURNAL OF PUBLIC HEALTH, 1996, 86 (08) :1142-1145

[26] ANTIBODY-DIRECT EPIFLUORESCENT FILTER TECHNIQUE FOR RAPID, DIRECT ENUMERATION OF ESCHERICHIA-COLI O157-H7 IN BEEF [J].

TORTORELLO, ML ;

STEWART, DS .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (10) :3553-3559

[27] FLUORESCENT-ANTIBODIES APPLIED TO DIRECT EPIFLUORESCENT FILTER TECHNIQUE FOR MICROSCOPIC ENUMERATION OF ESCHERICHIA-COLI O157-H7 IN MILK AND JUICE [J].

TORTORELLO, ML ;

GENDEL, SM .

JOURNAL OF FOOD PROTECTION, 1993, 56 (08) :672-677

[28] ISOLATION OF ESCHERICHIA-COLI SEROTYPE O157-H7 AND OTHER SHIGA-LIKE-TOXIN-PRODUCING ESCHERICHIA-COLI FROM DAIRY-CATTLE [J].

WELLS, JG ;

SHIPMAN, LD ;

GREENE, KD ;

SOWERS, EG ;

GREEN, JH ;

CAMERON, DN ;

DOWNES, FP ;

MARTIN, ML ;

GRIFFIN, PM ;

OSTROFF, SM ;

POTTER, ME ;

TAUXE, RV ;

WACHSMUTH, IK .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (05) :985-989

[29] Immunomagnetic-electrochemiluminescent detection of Escherichia coli O157 and Salmonella typhimurium in foods and environmental water samples [J].

Yu, H ;

Bruno, JG .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (02) :587-592