Microfluidic device for label-free measurement of platelet activation

被引：71

作者：

Inglis, David W. ^{[1
]}

Morton, Keith J. ^{[2
,3
]}

Davis, John A. ^{[2
,3
]}

Zieziulewicz, Thomas. J. ^{[4
]}

Lawrence, David A. ^{[4
]}

Austin, Robert H. ^{[5
]}

Sturm, James C. ^{[2
,3
]}

机构：

[1] Macquarie Univ, Dept Phys, Sydney, NSW 2109, Australia

[2] Princeton Univ, Dept Elect Engn, Princeton, NJ 08544 USA

[3] Macquarie Univ, PRISM, Sydney, NSW 2109, Australia

[4] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA

[5] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA

来源：

LAB ON A CHIP | 2008年 / 8卷 / 06期

关键词：

D O I：

10.1039/b800721g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work we demonstrate a new microfluidic method for the rapid assessment of platelet size and morphology in whole blood. The device continuously fractionates particles according to size by displacing them perpendicularly to the fluid flow direction in a micro-fabricated post array. Whole blood, labeled with the fluorescent, platelet specific, antibody PE-anti-CD41, was run through the device and the positions of fluorescent objects noted as they exited the array. From this, histograms of platelet size were created which show marked increases in size after exposure to thrombin or a temperature of 4 degrees C. We infer that the well known morphological changes that occur during activation are causing the observed increase in size.

引用

页码：925 / 931

页数：7

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