Deterministic hydrodynamics: Taking blood apart

被引：500

作者：

Davis, John A.

Inglis, David W.

Morton, Keith J.

Lawrence, David A.

Huang, Lotien R.

Chou, Stephen Y.

Sturm, James C.

Austin, Robert H. ^{[1
]}

机构：

[1] Princeton Univ, Princeton Inst Sci & Technol Mat, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Elect Engn, Princeton, NJ 08544 USA

[3] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA

[4] Wadsworth Ctr, Albany, NY 12201 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2006年 / 103卷 / 40期

关键词：

cells; plasma; separation; microfabrication;

D O I：

10.1073/pnas.0605967103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We show the fractionation of whole blood components and isolation of blood plasma with no dilution by using a continuous-flow deterministic array that separates blood components by their hydrodynamic size, independent of their mass. We use the technology we developed of deterministic arrays which separate white blood cells, red blood cells, and platelets from blood plasma at flow velocities of 1,000 mu m/sec and volume rates up to 1 mu l/min. We verified by flow cytometry that an array using focused injection removed 100% of the lymphocytes and monocytes from the main red blood cell and platelet stream, Using a second design, we demonstrated the separation of blood plasma from the blood cells (white, red, and platelets) with virtually no dilution of the plasma and no cellular contamination of the plasma.

引用

页码：14779 / 14784

页数：6

共 15 条

[1]

BOYUM A, 1974, TISSUE ANTIGENS, V4, P269

[2]

Dacie J. V., 2001, PRACTICAL HAEMATOLOG, V9, P633

[3] Parallel microchannel-based measurements of individual erythrocyte areas and volumes [J].