Solid state NMR for determination of degree of acetylation of chitin and chitosan

被引:277
作者
Heux, L
Brugnerotto, J
Desbrières, J
Versali, MF
Rinaudo, M
机构
[1] Univ Grenoble 1, CNRS, Ctr Rech Macromol Vegetales, F-38041 Grenoble 9, France
[2] Univ Liege, Dept Bot B22, Chitin Chitosan Res Grp, B-4000 Liege, Belgium
关键词
D O I
10.1021/bm000070y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The degree of acetylation (DA) of various samples has been evaluated by H-1 liquid-state NMR and C-13 and N-15 CP-MAS solid-state NMR over the whole range of DA. A good agreement has been found for all the experiments. The C-13 and N-15 CP-MAS experiments have permitted the evaluation of the chitin DA and content in the structural polysaccharides in a fungus named Aspergillus niger. The fungus structural carbohydrates mainly consist in pure chitin associated with glucans. Comparison of the C-13 CP-MAS spectra with standard (1-->3)-beta -D-glucans strongly suggests that chitin and glucans are linked via covalent bonds.
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收藏
页码:746 / 751
页数:6
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