Gene structure and expression of phospholemman in mouse

被引:21
作者
Bogaev, RC
Jia, LG
Kobayashi, YM
Palmer, CJ
Mounsey, JP
Moorman, JR
Jones, LR
Tucker, AL
机构
[1] Univ Virginia, Hlth Sci Ctr, Dept Internal Med, Div Cardiovasc, Charlottesville, VA 22908 USA
[2] Univ Virginia, Hlth Sci Ctr, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22908 USA
[3] Univ Virginia, Hlth Sci Ctr, Cardiovasc Res Ctr, Charlottesville, VA 22908 USA
[4] Indiana Univ, Sch Med, Krannert Inst Cardiol, Dept Med, Indianapolis, IN 46202 USA
关键词
chloride channel; channel regulator; taurine; library screening; northern blotting; alternative splicing;
D O I
10.1016/S0378-1119(01)00497-8
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Phospholemman (PLM) is a small transmembrane cardiac protein that is the major sarcolemmal substrate for phosphorylation in response to adrenergic stimulation. PLM likely plays a role in muscle contractility and cell volume regulation through its function as a channel or a channel regulator. We are the first to describe the structure of the PLM gene and to demonstrate PLM cDNA splice variants. We cloned the murine PLM cDNA and used it as a probe to isolate the gene from a 129/SvJ genomic library. The gene contains seven introns and eight exons. The coding sequence is interrupted by five introns; the 5 ' untranslated region by two. Using rapid amplification of 5 ' cDNA ends we identified transcription start sites and four splice variants of the 5 ' untranslated domain. There was no TATA box or CAAT box in the putative promoter regions. The gene has several stretches of dinucleotide repeats. The 3 ' untranslated domains of mouse PLM cDNA clones show sequence differences not accounted for by alternative splicing. Mouse PLM shares 93, 83 and 80% amino acid identity with rat, dog, and human PLMs, respectively. Tissue expression of murine PLM parallels that in other species, being highest in heart, skeletal muscle, and liver. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:69 / 79
页数:11
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