In vitro assays of vesicular transport

被引：14

作者：

Cook, NR ^{[1
]}

Davidson, HW ^{[1
]}

机构：

[1] Univ Cambridge, Addenbrookes Hosp, Wellcome Trust, Ctr Mol Mechanisms Dis, Cambridge CB2 2XY, England

来源：

TRAFFIC | 2001年 / 2卷 / 01期

关键词：

broken cell; cell free; membrane fusion; vesicle budding; vesicular transport;

D O I：

10.1034/j.1600-0854.2001.020104.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Movement of proteins and lipids between the various compartments of eukaryotic cells is fundamental to the maintenance of cellular homeostasis. and an understanding of the molecular mechanisms that govern these processes remains a key goal of cell biological research. This aim has been greatly facilitated by the development of assays that recapitulate specific events in vitro. In the following article we provide an overview of some of the currently used assays that measure the movement of proteins within the exocytic and endocytic pathways, and provide a starting point for those wishing to establish their own systems to study other vesicular transport steps.

引用

页码：19 / 25

页数：7

共 83 条

[11] SEMI-INTACT CELLS PERMEABLE TO MACROMOLECULES - USE IN RECONSTITUTION OF PROTEIN-TRANSPORT FROM THE ENDOPLASMIC-RETICULUM TO THE GOLGI-COMPLEX [J].