Silanized surfaces for in vitro studies of actomyosin function and nanotechnology applications

被引:71
作者
Sundberg, M
Rosengren, JP
Bunk, R
Lindahl, J
Nicholls, IA
Tågerud, S
Omling, P
Montelius, L
Månsson, A
机构
[1] Univ Kalmar, Dept Chem & Biomed Sci, SE-39182 Kalmar, Sweden
[2] Lund Univ, Div Solid State Phys, SE-22100 Lund, Sweden
[3] Lund Univ, Nanometer Consortium, SE-22100 Lund, Sweden
关键词
heavy meromyosin; trimethylehlorosilane; octadecyltrichlorosilane; dimethyldichlorosilane; nitrocellulose; actomyosin; motor protein; molecular motor;
D O I
10.1016/j.ab.2003.07.022
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We have previously shown that selective heavy meromyosin (HMM) adsorption to predefined regions of nanostructured polymer resist surfaces may be used to produce a nanostructured in vitro motility assay. However, actomyosin function was of lower quality than on conventional nitrocellulose films. We have therefore studied actomyosin function on differently derivatized glass surfaces with the aim to find a substitute for the polymer resists. We have found that surfaces derivatized with trimethylchlorosilane (TMCS) were superior to all other surfaces tested, including nitrocellulose. High-quality actin filament motility was observed up to 6 days after incubation with HMM and the fraction of motile actin filaments and the velocity of smooth sliding were generally higher on TMCS than on nitrocellulose. The actomyosin function on TMCS-derivatized glass and nitrocellulose is considered in relation to roughness and hydrophobicity of these surfaces. The results suggest that TMCS is an ideal substitute for polymer resists in the nanostructured in vitro motility assay. Furthermore, TMCS derivatized glass also seems to offer several advantages over nitrocellulose for HMM adsorption in the ordinary in vitro motility assay. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:127 / 138
页数:12
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