Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples

被引：55

作者：

Fukano, T ^{[1
]}

Miyawaki, A ^{[1
]}

机构：

[1] Inst Brain Sci, Inst Phys & Chem Res, Adv Technol Dev Grp, Lab Cell Funct & Dynam, Wako, Saitama 3510198, Japan

来源：

APPLIED OPTICS | 2003年 / 42卷 / 19期

关键词：

D O I：

10.1364/AO.42.004119

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have developed a whole-field fluorescence microscope equipped with a Digital Micromirror Device to acquire optically sectioned images by using the fringe-projection technique and the phase-shift method. This system allows free control of optical sectioning strength through computer-controlled alteration of the fringe period projected onto a sample. We have employed this system to image viable cells expressing fluorescent proteins and discussed its biological applications. (C) 2003 Optical Society of America.

引用

页码：4119 / 4124

页数：6

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