The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors

被引：166

作者：

Schaefer-Klein, J

Givol, I

Barsov, EV

Whitcomb, JM

VanBrocklin, M

Foster, DN

Federspiel, MJ

Hughes, SH

机构：

[1] NCI, Frederick Canc Res & Dev Ctr, Retroviral Replicat & Vector Design Sect, ABL Basic Res Program, Frederick, MD 21702 USA

[2] Mayo Clin & Mayo Fdn, Program Mol Med, Rochester, MN 55905 USA

[3] ViroLog Inc, S San Francisco, CA 94080 USA

[4] Univ Minnesota, Dept Anim Sci, St Paul, MN 55108 USA

来源：

VIROLOGY | 1998年 / 248卷 / 02期

关键词：

D O I：

10.1006/viro.1998.9291

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The lack of a well-behaved permanent, adherent, nontransformed chicken cell line has made some experiments with avian leukosis-sarcoma viruses (ASLV) and vectors considerably more difficult. The EV-O-derived line, DF-I, supports the efficient replication of subgroups (A), (B), and (C) ASLV, as well as amphotrophic murine leukemia virus and an ASLV-derived vector that has its env gene derived from the env gene from an amphotrophic murine leukemia virus. The cell line responds appropriately to the expression of a transforming oncogene (v-myc) to a growth suppressor gene [p21(wail)] and can be sorted (using FAGS) ii infected by an ASLV vector that expresses GFP. (C) 1998 Academic Press.

引用

页码：305 / 311

页数：7

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