Identification of piRNAs in Hela cells by massive parallel sequencing

被引：58

作者：

Lu, Yilu

Li, Chao

Zhang, Kun

Sun, Huaqin

Tao, Dachang

Liu, Yunqiang

Zhang, Sizong

Ma, Yongxin ^{[1
]}

机构：

[1] Sichuan Univ, Dept Med Genet, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Peoples R China

来源：

BMB REPORTS | 2010年 / 43卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Hela; piRNA; Piwi; SMALL RNAS; DNA METHYLATION; FAMILY-MEMBERS; PROTEIN PIWIL2; C-ELEGANS; GERMLINE; PATHWAY; DROSOPHILA; TESTES; RETROTRANSPOSON;

D O I：

10.3858/BMBRep.2010.43.9.635

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Piwi proteins and Piwi-interacting RNAs (piRNAs) have been implicated in transposon control in germline from Drosophila to mammals. To examine the profile of small RNA expression in human cancer cells and explore difference in small RNA transcriptome, small RNA libraries prepared from wildtype, HILI overexpressed and HILI knockdowned Hela cells were sequenced using Solexa technology. piRNAs and other repeat-associated small RNAs were observed in Hela cells. By using in situ hybridization, piR-49322 was localized in the nucleolus and around the periphery of nuclear membrane in Hela cells. Following the overexpression of HILI, the retro-transposon elements LINE1 was significantly repressed, while LINE1-associated small RNAs decreased in abundance. The present study demonstrated that HILI along with piRNAs plays a role in LINE1 suppression in Hela cancer cell line. [BMB reports 2010; 43(9): 635-641]

引用

收藏

页码：635 / 641

页数：7

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