Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography

被引：187

作者：

Dalby, MJ

Giannaras, D

Riehle, MO

Gadegaard, N

Affrossman, S

Curtis, ASG

机构：

[1] Univ Glasgow, Inst Biomed & Life Sci, Ctr Cell Engn, Glasgow G12 8QQ, Lanark, Scotland

[2] Univ Strathclyde, Dept Pure & Appl Chem, Glasgow G1 1XL, Lanark, Scotland

来源：

BIOMATERIALS | 2004年 / 25卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

nano-topography; fibroblasts; cytoskeleton; cell adhesion;

D O I：

10.1016/S0142-9612(03)00475-7

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical method of producing nano-topography. This manuscript investigates human fibroblast response to 27 nm high nano-islands produced by polymer demixing. Cell spreading, cytoskeleton, focal adhesion and Rac localisation were studied. The results showed that an initial rapid adhesion and cytoskeletal formation on the islands at 4 days of culture gave way to poorly formed contacts and vimentin cytoskeleton at 30 days of culture. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：77 / 83

页数：7

共 24 条

[1] The effect of molecular weight an the topography of thin films of blends of poly(4-bromostyrene) and polystyrene [J].