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Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography

被引：187
作者：
Dalby, MJ
Giannaras, D
Riehle, MO
Gadegaard, N
Affrossman, S
Curtis, ASG
机构：
[1] Univ Glasgow, Inst Biomed & Life Sci, Ctr Cell Engn, Glasgow G12 8QQ, Lanark, Scotland
[2] Univ Strathclyde, Dept Pure & Appl Chem, Glasgow G1 1XL, Lanark, Scotland
来源：
BIOMATERIALS | 2004年 / 25卷 / 01期
基金：
英国生物技术与生命科学研究理事会;
关键词：
nano-topography; fibroblasts; cytoskeleton; cell adhesion;
D O I：
10.1016/S0142-9612(03)00475-7
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical method of producing nano-topography. This manuscript investigates human fibroblast response to 27 nm high nano-islands produced by polymer demixing. Cell spreading, cytoskeleton, focal adhesion and Rac localisation were studied. The results showed that an initial rapid adhesion and cytoskeletal formation on the islands at 4 days of culture gave way to poorly formed contacts and vimentin cytoskeleton at 30 days of culture. (C) 2003 Elsevier Ltd. All rights reserved.
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收藏
页码：77 / 83
页数：7
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