Evolutionary conservation of kinetochore protein sequences in plants

被引：29

作者：

ten Hoopen, R ^{[1
]}

Manteuffel, R

Dolezel, J

Malysheva, L

Schubert, I

机构：

[1] Inst Plant Genet & Crop Plant Res IPK, D-06466 Gatersleben, Germany

[2] Inst Expt Bot, Olomouc 77200, Czech Republic

来源：

CHROMOSOMA | 2000年 / 109卷 / 07期

关键词：

D O I：

10.1007/s004120000109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The evolutionary conservation of structural/functional kinetochore proteins has been studied on isolated nuclei and pro-/metaphase chromosomes of mono- and dicot plants. The cross-reactivities of antibodies against human CENPC, CENPE and CENPF and against maize CENPCa with the centromeric regions of mitotic chromosomes of Vicia faba and/or Hordeum vulgare are shown. Putative homologs of the kinetochore protein SKP1 (suppressor of kinetochore protein 1p of yeast) were found in both species and of CBF5p (centromere binding factor 5 of yeast) in barley. Antibodies against synthetic peptides derived from partial sequences encoding these proteins were produced and recognized the centromeric regions on mitotic chromosomes as detected by indirect immunofluorescence.

引用

页码：482 / 489

页数：8

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