Evolutionary conservation of kinetochore protein sequences in plants

被引：29

作者：

ten Hoopen, R ^{[1
]}

Manteuffel, R

Dolezel, J

Malysheva, L

Schubert, I

机构：

[1] Inst Plant Genet & Crop Plant Res IPK, D-06466 Gatersleben, Germany

[2] Inst Expt Bot, Olomouc 77200, Czech Republic

来源：

CHROMOSOMA | 2000年 / 109卷 / 07期

关键词：

D O I：

10.1007/s004120000109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The evolutionary conservation of structural/functional kinetochore proteins has been studied on isolated nuclei and pro-/metaphase chromosomes of mono- and dicot plants. The cross-reactivities of antibodies against human CENPC, CENPE and CENPF and against maize CENPCa with the centromeric regions of mitotic chromosomes of Vicia faba and/or Hordeum vulgare are shown. Putative homologs of the kinetochore protein SKP1 (suppressor of kinetochore protein 1p of yeast) were found in both species and of CBF5p (centromere binding factor 5 of yeast) in barley. Antibodies against synthetic peptides derived from partial sequences encoding these proteins were produced and recognized the centromeric regions on mitotic chromosomes as detected by indirect immunofluorescence.

引用

页码：482 / 489

页数：8

共 40 条

[21] Mapping of repeated DNA sequences in plant chromosomes by PRINS and C-PRINS [J].

Kubalakova, M ;

Macas, J ;

Dolezel, J .

THEORETICAL AND APPLIED GENETICS, 1997, 94 (6-7) :758-763

[22]

KUNZEL G, 1979, BIOL ZBL, V98, P587

[23] A 240-KD MULTISUBUNIT PROTEIN COMPLEX, CBF3, IS A MAJOR COMPONENT OF THE BUDDING YEAST CENTROMERE [J].

LECHNER, J ;

CARBON, J .

CELL, 1991, 64 (04) :717-725

[24] CENP-F IS A PROTEIN OF THE NUCLEAR MATRIX THAT ASSEMBLES ONTO KINETOCHORES AT LATE G2 AND IS RAPIDLY DEGRADED AFTER MITOSIS [J].

LIAO, H ;

WINKFEIN, RJ ;

MACK, G ;

RATTNER, JB ;

YEN, TJ .

JOURNAL OF CELL BIOLOGY, 1995, 130 (03) :507-518

[25] ANTIBODIES TO THE KINESIN MOTOR DOMAIN AND CENP-E INHIBIT MICROTUBULE DEPOLYMERIZATION-DEPENDENT MOTION OF CHROMOSOMES IN-VITRO [J].

LOMBILLO, VA ;

NISLOW, C ;

YEN, TJ ;

GELFAND, VI ;

MCINTOSH, JR .

JOURNAL OF CELL BIOLOGY, 1995, 128 (1-2) :107-115

[26]

Maney T, 2000, INT REV CYTOL, V194, P67

[27] Amplification of cDNA ends based on template-switching effect and step-out PCR [J].

Matz, M ;

Shagin, D ;

Bogdanova, E ;

Britanova, O ;

Lukyanov, S ;

Diatchenko, L ;

Chenchik, A .

NUCLEIC ACIDS RESEARCH, 1999, 27 (06) :1558-1560

[28] The Nop60B gene of Drosophila encodes an essential nucleolar protein that functions in yeast [J].

Phillips, B ;

Billin, AN ;

Cadwell, C ;

Buchholz, R ;

Erickson, C ;

Merriam, JR ;

Carbon, J ;

Poole, SJ .

MOLECULAR AND GENERAL GENETICS, 1998, 260 (01) :20-29

[29] Arabidopsis SKP1, a homologue of a cell cycle regulator gene, is predominantly expressed in meristematic cells [J].

Porat, R ;

Lu, PZ ;

O'Neill, SD .

PLANTA, 1998, 204 (03) :345-351

[30] A TY3/GYPSY retrotransposon-like sequence localizes to the centromeric regions of cereal chromosomes [J].

Presting, GG ;

Malysheva, L ;

Fuchs, J ;

Schubert, IZ .

PLANT JOURNAL, 1998, 16 (06) :721-728

← 1 2 3 4 →