Floc stabilization for multiple microscopic techniques

被引:56
作者
Droppo, IG
Flannigan, DT
Leppard, GG
Jaskot, C
Liss, SN
机构
[1] MCMASTER UNIV, DEPT BIOL, HAMILTON, ON L8S 4K1, CANADA
[2] RYERSON POLYTECH UNIV, DEPT APPL CHEM & BIOL SCI, TORONTO, ON M5B 2K3, CANADA
关键词
D O I
10.1128/AEM.62.9.3508-3515.1996
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A nondestructive stabilization technique for the characterization of microbial flocs which permits the application of correlative microscopic techniques is described. Flocs embedded in agarose are retained in a porous, resilient medium which allows for the transport, staining, washing, and subsampling of the flocculated material directly within a plankton chamber with minimal or no destructive forces. A single agarose disc can be subdivided into numerous sections for analysis by several microscope types and associated techniques.
引用
收藏
页码:3508 / 3515
页数:8
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