Optimization of temperature and initial pH and kinetic analysis of tartaric acid production by Gluconobacter suboxydans
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作者:
Chandrashekar, K
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Indian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Madras 600036, Chennai, IndiaIndian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Madras 600036, Chennai, India
Chandrashekar, K
[1
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Felse, PA
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Indian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Madras 600036, Chennai, IndiaIndian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Madras 600036, Chennai, India
Felse, PA
[1
]
Panda, T
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Indian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Madras 600036, Chennai, IndiaIndian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Madras 600036, Chennai, India
Panda, T
[1
]
机构:
[1] Indian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Madras 600036, Chennai, India
Response surface methodology was applied for the simultaneous optimization of initial pH and temperature of fermentation for the production of tartaric acid by Gluconobacter suboxydans. A temperature of 32.8 degrees C and an initial pH of 6.05 was found to be most suitable. Kinetic analysis for the growth of Gluconobacter suboxydans and product formation was done and a logistic model was used to describe the growth of Gluconobacter suboxydans while the Leudeking-Piret model explained the product formation. Parameters of the model were evaluated and tartaric acid formation was found to be non-growth associated.