DNA analysis at p53 locus in carcinomas arising from pleomorphic adenomas of salivary glands: Comparison of molecular study and p53 immunostaining

被引:31
作者
Yamamoto, Y
Kishimoto, Y
Wistuba, II
Virmani, AK
Vuitch, F
Gazdar, AF
Albores-Saavedra, J
机构
[1] Osaka Med Coll, Dept Otolaryngol, Takatsuki, Osaka 569, Japan
[2] Univ Texas, SW Med Ctr, Dept Pathol, Dallas, TX USA
[3] Univ Texas, SW Med Ctr, Hamon Ctr Therapeut Oncol Res, Dallas, TX USA
关键词
immunohistochemistry; loss of heterozygosity; p53; gene; pleomorphic adenoma; salivary gland carcinoma;
D O I
10.1111/j.1440-1827.1998.tb03904.x
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Where and how frequently p53 abnormalities are involved in the development of pleomorphic adenoma (PA) and its malignant progression to carcinoma was investigated. The presence of p53 gene abnormalities was analyzed in eight patients with carcinoma in pleomorphic adenoma (CPA) by polymerase chain reaction (PCR)-based assays and immunohistochemistry. Normal salivary gland tissue, adenomatous, transitional and carcinomatous areas were microdissected from archival microslides and analyzed for allelic deletions of the p53 gene using two microsatellite markers at the p53 locus; dinucleotide (CA)(n) repeat and pentanucleotide (AAAAT)(n) repeat. Loss of heterozygosity (LOH) of the p53 gene was detected in 57% of adenomas, 86% of transitional lesions and 86% of carcinomas. In contrast, overexpression of p53 oncoprotein was noted immunohistochemically in 13% of adenomas, 50% of transitional areas and 75% of carcinomas. All of the tumors with immunoreactivity for p53 oncoprotein demonstrated LOH, Moreover, when LOH was present in adenomatous or transitional areas, the identical LOH was always detected in the corresponding carcinomatous areas in the same CPA tumors. These findings indicate that p53 gene mutation is an early event and occurs frequently at an early stage of precancerous lesions and may be responsible for most cases of malignant transformation of PA.
引用
收藏
页码:265 / 272
页数:8
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