Enzymes which are stable in the presence of organic solvents

被引：281

作者：

Ogino, H ^{[1
]}

Ishikawa, H ^{[1
]}

机构：

[1] Univ Osaka Prefecture, Dept Chem Engn, Sakai, Osaka 5998531, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2001年 / 91卷 / 02期

关键词：

organic solvent-stable enzyme; stabilization; modification;

D O I：

10.1263/jbb.91.109

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

There are numerous advantages of employing enzymes as catalysts in organic solvents or aqueous solutions containing organic solvents instead of water. A few natural enzymes which are stable in the presence of organic solvents have been discovered. However, almost all natural enzymes are easily denatured and inactivated in the presence of organic solvents. Therefore, several physical and chemical methods, such as immobilization, modification, and entrapment, for stabilizing enzymes in the presence of organic solvents were developed. Protein engineering using site directed mutagenesis and directed evolution are useful for clarifying why organic solvent-stable enzymes are stable in the presence of organic solvents and for developing organic solvent-stable mutant enzymes.

引用

页码：109 / 116

页数：8

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