Identification of putative Arabidopsis DEMETER target genes by GeneChip analysis

被引：32

作者：

Ohr, Hyonhwa

Bui, Anhthu Q.

Le, Brandon H.

Fischer, Robert L.

Choi, Yeonhee ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Sci Biol, Seoul 151742, South Korea

[2] Univ Calif Los Angeles, Cell & Dev Biol, Dept Mol, Los Angeles, CA 90095 USA

[3] Univ Calif Berkeley, Dept Plant & Micriobial Biol, Berkeley, CA 94720 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 364卷 / 04期

关键词：

gene imprinting; DNA demethylation; DNA glycosylase; GeneChip analysis;

D O I：

10.1016/j.bbrc.2007.10.092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The Arabidopsis DEMETER (DME) DNA glycosylase is required for the maternal allele expression of imprinted Polycomb group (MEDEA and FIS2) and transcription factor (FWA) genes in the endosperm. Expression of DME in the central cell, not in pollen or stamen, establishes gene imprinting by hypomethylating maternal alleles. However, little is known about other genes regulated by DME. To identify putative DME target genes, we generated CaMV:DME plants which ectopically express DME in pollen and stamens. Comparison of mRNA profiles revealed 94 genes induced by ectopic DME expression in both stamen and pollen. Gene ontology analysis identified three molecular functions enriched in the DME-inducible RNA list: DNA or RNA binding, kinase activity, and transcription factor activity. Semi-quantitative RT-PCR verified the candidate genes identified by GeneChip analysis. The putative target genes identified in this study will provide insights into the regulatory mechanism of DME DNA glycosylase and the functions of DNA demethylation. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：856 / 860

页数：5

共 31 条

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Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation [J].