Spatial mapping of nitric oxide generation in the ischemic heart using electron paramagnetic resonance imaging

被引:37
作者
Kuppusamy, P
Wang, PH
Samouilov, A
Zweier, JL
机构
[1] JOHNS HOPKINS MED INST,DEPT MED,DIV CARDIOL,MOL & CELLULAR BIOPHYS LABS,BALTIMORE,MD 21205
[2] JOHNS HOPKINS MED INST,EPR CTR,BALTIMORE,MD 21205
关键词
EPR imaging; rat heart; ischemia; nitric oxide; nitrite; acidosis;
D O I
10.1002/mrm.1910360207
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Recently, it has been shown that rat hearts subjected to global ischemia generate nitric oxide (NO) and that a significant portion of it is generated by the reduction of nitrite under the acidic and reducing conditions that occur during myocardial ischemia [Zweier, Wang, Samouilov, Kuppusamy, Nature Med. 1, 804-809 (1995)]. In the present study it is further attempted to map the spatial distributions of the NO generation in the ischemic myocardium using L-band electron paramagnetic resonance imaging. Rat hearts were loaded with 10 mM nitrite and subjected to global no-flow ischemia, during which time a series of three-dimensional spatial electron paramagnetic resonance (EPR) images of the distribution of NO were obtained using the NO trap bis(N-methyl-D-glucamine dithiocarbamate)iron(II). The images clearly showed that NO is formed throughout the myocardium. Kinetic experiments showed that maximum NO generation and trapping occur at the midmyocardium and spread out to endocardium and epicardium of the left ventricle. The magnitude of generation in the RV myocardium is four- to fivefold lower than in the LV.
引用
收藏
页码:212 / 218
页数:7
相关论文
共 39 条
[1]   APPARENT HYDROXYL RADICAL PRODUCTION BY PEROXYNITRITE - IMPLICATIONS FOR ENDOTHELIAL INJURY FROM NITRIC-OXIDE AND SUPEROXIDE [J].
BECKMAN, JS ;
BECKMAN, TW ;
CHEN, J ;
MARSHALL, PA ;
FREEMAN, BA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1620-1624
[2]  
BECKMAN JS, 1991, J DEV PHYSIOL, V15, P53
[3]   DEVELOPMENT OF AN ELECTRONICALLY TUNABLE L-BAND RESONATOR FOR EPR SPECTROSCOPY AND IMAGING OF BIOLOGICAL SAMPLES [J].
CHZHAN, M ;
KUPPUSAMY, P ;
ZWEIER, JL .
JOURNAL OF MAGNETIC RESONANCE SERIES B, 1995, 108 (01) :67-72
[4]   AN OPTIMIZED L-BAND CERAMIC RESONATOR FOR EPR IMAGING OF BIOLOGICAL SAMPLES [J].
CHZHAN, M ;
SHTEYNBUK, M ;
KUPPUSAMY, P ;
ZWEIER, JL .
JOURNAL OF MAGNETIC RESONANCE SERIES A, 1993, 105 (01) :49-53
[5]  
Dawson T.M., 1994, NEUROSCIENTIST, V1, P9
[6]  
DAWSON VL, 1993, J NEUROSCI, V13, P2651
[7]   CHEMICAL GENERATION OF NITRIC-OXIDE IN THE MOUTH FROM THE ENTEROSALIVARY CIRCULATION OF DIETARY NITRATE [J].
DUNCAN, C ;
DOUGALL, H ;
JOHNSTON, P ;
GREEN, S ;
BROGAN, R ;
LEIFERT, C ;
SMITH, L ;
GOLDEN, M ;
BENJAMIN, N .
NATURE MEDICINE, 1995, 1 (06) :546-551
[8]  
Ewert U., 1991, EPR IMAGING IN VIVO, P119
[9]   UNUSUALLY STABLE THIONITRITE FROM N-ACETYL-D,L-PENICILLAMINE - X-RAY CRYSTAL AND MOLECULAR-STRUCTURE OF 2-(ACETYLAMINO)-2-CARBOXY-1,1-DIMETHYLETHYL THIONITRITE [J].
FIELD, L ;
DILTS, RV ;
RAVICHANDRAN, R ;
LENHERT, PG ;
CARNAHAN, GE .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1978, (06) :249-250
[10]   ENDOTHELIUM-DERIVED RELAXING AND CONTRACTING FACTORS [J].
FURCHGOTT, RF ;
VANHOUTTE, PM .
FASEB JOURNAL, 1989, 3 (09) :2007-2018