Quantification of water-soluble vitamins in milk-based infant formulae using biosensor-based assays

被引：58

作者：

Gao, Yanling ^{[1
]}

Guo, Fei ^{[2
]}

Gokavi, Sumangala ^{[1
]}

Chow, Andrew ^{[2
]}

Sheng, Qinghai ^{[3
]}

Guo, Mingruo ^{[1
]}

机构：

[1] Univ Vermont, Dept Nutr & Food Sci, Burlington, VT 05405 USA

[2] GE Healthcare Biosci, Picataway, NJ USA

[3] Shijiazhuang Sanlu Grp, Shijiazhuang 050071, Peoples R China

来源：

FOOD CHEMISTRY | 2008年 / 110卷 / 03期

关键词：

water-soluble vitamins; quantification; biosensor; infant formula;

D O I：

10.1016/j.foodchem.2008.03.007

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Vitamin analysis is essential for quality control and development of functional foods. in this study, a biosensor-based technology developed by Biacore AB was evaluated for analysis of water-soluble vitamins B-2, B-12, folic acid, biotin, and pantothenic acid used to supplement infant formula samples. Performance parameters such as accuracy, repeatability and recovery for the five vitamins were studied. The repeatability was measured in terms of relative standard deviation (RSD) and HORRAT(r) value. The RSD for all vitamins was below 2% and the values of HORRAT(r), were 0.16, 0.10, 0.15, 0.11 and 0.22, for B2, B12, folic acid, biotin, and pantothenic acid, respectively. The recovery of vitamins ranged from 94.7% to 109.1%. Linear analyses indicated that the square of the correlation coefficient (R-2) for B-2, B-12, folic acid, biotin, and pantothenic acid were 0.993, 0.997, 0.993, 0.993 and 0.995, respectively. The results showed that the biosensor-based vitamin analysis technology is a sensitive, reliable and realistic alternative to other methods. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：769 / 776

页数：8

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