Inhibition of in vitro pre-mRNA splicing in S. cerevisiae by branched oligonucleotides

被引：7

作者：

Carriero, S

Braich, RS

Hudson, RHE

Anglin, D

Friesen, JD

Damha, MJ

机构：

[1] McGill Univ, Dept Chem, Montreal, PQ H3A 2K6, Canada

[2] Univ Toronto, Dept Med Res, Banting & Best Inst, Toronto, ON M5G 1L6, Canada

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1081/NCN-100002449

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of V- and Y-shaped nucleic acids, related to the splicing intermediates derived from S. cerevisiae actin pre-mRNA, were prepared. The effects of such branched nucleic acids (bNAs) on the efficiency of in vitro pre-mRNA splicing in yeast were studied. The exogenous bNAs each effect the efficiency of splicing, yet to different degrees, depending on the sugar composition and topology of the molecules. Y-shaped RNAs inhibited the formation of mRNA (i.e. RNA splicing) to the greatest extent.

引用

收藏

页码：873 / 877

页数：5

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