SOLID-PHASE SYNTHESIS OF BRANCHED OLIGORIBONUCLEOTIDES RELATED TO MESSENGER-RNA SPLICING INTERMEDIATES

被引：57

作者：

DAMHA, MJ ^{[1
]}

GANESHAN, K ^{[1
]}

HUDSON, RHE ^{[1
]}

ZABARYLO, SV ^{[1
]}

机构：

[1] UNIV TORONTO,ERINDALE COLL,DEPT CHEM,MISSISSAUGA L5L 1C6,ONTARIO,CANADA

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 24期

关键词：

D O I：

10.1093/nar/20.24.6565

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chemical synthesis of oligoribonucleotides containing vicinal (2'-5')- and (3'-5')-phosphodiester linkages is described. The solid-phase method, based on silyl-phosphoramidite chemistry, was applied to the synthesis of a series of branched RNA [(Xp)(n)A2' (pN)n3'(pN)n] related to the splicing intermediates derived from Saccharomyces cerevisiae rp51a pre-messenger RNA. The branched oligonucleotides have been thoroughly characterized by nucleoside and branched nucleotide composition analysis. Branched oligoribonucleotides will be useful in the study of messenger RNA splicing and in determining the biological role of RNA 'lariats' and 'forks' in vivo.

引用

页码：6565 / 6573

页数：9

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