Deletion of a lectin gene does not affect the phenotype of the nematode-trapping fungus Arthrobotrys oligospora

被引:34
作者
Balogh, J [1 ]
Tunlid, A [1 ]
Rosén, S [1 ]
机构
[1] Lund Univ, Dept Microbial Ecol, SE-22362 Lund, Sweden
关键词
Arthrobotrys oligospora; saline-soluble lectin; nematode-trapping fungus; collembolan feeding; nitrogen storage;
D O I
10.1016/S1087-1845(03)00023-9
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A number of filamentous fungi are known to produce high levels of saline-soluble and low-molecular-mass lectins. The function of these proteins are not clear but it has been proposed that they are involved in storage of nutrients, development, recognition of other organisms, and defense reactions. A gene encoding such a lectin (AOL) was deleted in the nematode-trapping fungus Arthrobotrys oligospora by homologous recombination. The deletion mutants did not express any hemagglutinating activity or protein cross-reacting with AOL antibodies. There were no significant differences between the DeltaAOL and wild-type strains in spore (conidia) germination, saprophytic growth, and pathogenicity. Furthermore, there was no significant difference in the growth and reproduction of collembolan feeding on the various strains of A. oligospora. Thus either the previous proposed functions of AOL are not correct, or the fungus can compensate for the absence of the lectin by expressing other proteins with similar function(s) as AOL. (C) 2003 Published by Elsevier Science (USA).
引用
收藏
页码:128 / 135
页数:8
相关论文
共 37 条
[1]   Improving the pathogenicity of a nematode-trapping fungus by genetic engineering of a subtilisin with nematotoxic activity [J].
Åhman, J ;
Johansson, T ;
Olsson, M ;
Punt, PJ ;
van den Hondel, CAMJJ ;
Tunlid, A .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (07) :3408-3415
[2]  
[Anonymous], NUCL ACIDS RES
[3]   INFLUENCE OF FUNGAL FOOD QUALITY ON THE GROWTH AND FECUNDITY OF FOLSOMIA-CANDIDA (COLLEMBOLA, ISOTOMIDAE) [J].
BOOTH, RG ;
ANDERSON, JM .
OECOLOGIA, 1979, 38 (03) :317-323
[4]   ISOLATION AND PARTIAL CHARACTERIZATION OF A CARBOHYDRATE-BINDING PROTEIN FROM A NEMATODE-TRAPPING FUNGUS [J].
BORREBAECK, CAK ;
MATTIASSON, B ;
NORDBRINGHERTZ, B .
JOURNAL OF BACTERIOLOGY, 1984, 159 (01) :53-56
[5]   Fruiting body development in Coprinus cinereus:: regulated expression of two galectins secreted by a non-classical pathway [J].
Boulianne, RP ;
Liu, Y ;
Aebi, M ;
Lu, BC ;
Kües, U .
MICROBIOLOGY-SGM, 2000, 146 :1841-1853
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   The Gal/GalNAc-specific lectin from the plant pathogenic basidiomycete Rhizoctonia solani is a member of the ricin-B family [J].
Candy, L ;
Peumans, WJ ;
Menu-Bouaouiche, L ;
Astoul, CH ;
Van Damme, J ;
Van Damme, EJM ;
Erard, M ;
Rougé, P .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 282 (03) :655-661
[8]  
Carroll A.M., 1994, FUNGAL GENET NEWSL, V41, P22, DOI [DOI 10.4148/1941-4765.1367, 10.4148/19414765.1367, DOI 10.4148/19414765.1367, 10.4148/1941-4765.1367]
[9]   Fungal galectins, sequence and specificity of two isolectins from Coprinus cinereus [J].
Cooper, DNW ;
Boulianne, RP ;
Charlton, S ;
Farrell, EM ;
Sucher, A ;
Lu, BC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (03) :1514-1521
[10]   A novel subfamily of Hsp70s in the endoplasmic reticulum [J].
Craven, RA ;
Tyson, JR ;
Stirling, CJ .
TRENDS IN CELL BIOLOGY, 1997, 7 (07) :277-282