Comparative genome analysis of Vibrio vulnificus, a marine pathogen

被引:287
作者
Chen, CY
Wu, KM
Chang, YC
Chang, CH
Tsai, HC
Liao, TL
Liu, YM
Chen, HJ
Shen, ABT
Li, JC
Su, TL
Shao, CP
Lee, CT
Hor, LI
Tsai, SF [1 ]
机构
[1] Natl Hlth Res Inst, Div Mol & Genom Med, Taipei 115, Taiwan
[2] Ind Technol Res Inst, Ctr Biomed Engn, Hsinchu 310, Taiwan
[3] Natl Yang Ming Univ, Inst Genet, Taipei 112, Taiwan
[4] Natl Yang Ming Univ, Genom Res Ctr, Taipei 112, Taiwan
[5] Natl Cheng Kung Univ, Dept Microbiol & Immunol, Tainan 701, Taiwan
关键词
D O I
10.1101/gr.1295503
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The halophile Vibrio vulnificus is an etiologic agent of human mortality from seafood-borne infections. We applied whole-genome sequencing and comparative analysis to investigate the evolution of this pathogen. The genome of biotype I strain, V. vulniflicus YJ016, was sequenced and includes two chromosomes of estimated 3377 kbp and 1857 kbp in size, and a plasmid-of 48,508 bp. A super-integron (SI) was identified, and the SI region spans 139 kbp and contains 188 gene cassettes. In contrast to non-SI sequences, the captured gene cassettes are unique for any given Vibrio species and are highly variable among V. vulnificus strains. Multiple rearrangements were found when comparing the 5.3-Mbp V. vulnificus YJ016 genome and the 4.0-Mbp V. cholerae El Tor N16961 genome. The organization of gene clusters of capsular polysaccharide, iron metabolism, and RTX toxin showed distinct genetic features of V Vulnificus and V. cholerae. The content of the V vulnificus genome contained gene duplications and evidence of horizontal transfer, allowing for genetic diversity and function in the marine environment. The genomic information obtained in this study can be applied to monitoring vibrio infections and identifying virulence genes in V vulnificus.
引用
收藏
页码:2577 / 2587
页数:11
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