A comparison of sequence resolution on plant chromosomes: PRINS versus FISH

被引：22

作者：

Menke, M ^{[1
]}

Fuchs, J ^{[1
]}

Schubert, I ^{[1
]}

机构：

[1] IPK, Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany

来源：

THEORETICAL AND APPLIED GENETICS | 1998年 / 97卷 / 08期

关键词：

plant chromosomes; primed in situ; DNA labelling; fluorescent in situ hybridization; repetitive sequences;

D O I：

10.1007/s001220051024

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The resolution of the chromosomal positions of six high- and one low-copy sequences by oligonucleotide-primed in situ (PRINS) labelling was compared with corresponding data obtained after fluorescent in situ hybridization (FISH) on field-bean and barley chromosomes. While PRINS proved to be suitable for the rapid detection of high-copy tandem repeats: at the same loci as those revealed by FISH, no clear PRINS signal was obtained for the low-copy family of vicilin genes at their locus on field-bean chromosome II. This indicates that localization of short target sequences by primer extension via Taq polymerase in situ does not yet provide a resolution equal, or superior, to FISH on plant chromosomes. Therefore, the use of a cocktail of chromosome-specific single-copy sequences as primers for PRINS is no alternative for the not as yet feasible chromosome painting in plants.

引用

页码：1314 / 1320

页数：7

共 44 条

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