Photolithographic synthesis of high-density oligonucleotide probe arrays

被引：32

作者：

Barone, AD ^{[1
]}

Beecher, JE ^{[1
]}

Bury, PA ^{[1
]}

Chen, C ^{[1
]}

Doede, T ^{[1
]}

Fidanza, JA ^{[1
]}

McGall, GH ^{[1
]}

机构：

[1] Affymetrix Inc, Santa Clara, CA 95051 USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

关键词：

D O I：

10.1081/NCN-100002328

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-density DNA probe arrays provide a massively parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research and diagnostics. Light-directed combinatorial oligonucleotide synthesis has enabled the large-scale production of GeneChip(R) probe arrays which contain several hundred of thousand oligonucleotide sequences on glass "chips" about one cm(2) in size. Due to their very high information content, GeneChip(R) probe arrays are finding widespread use in the hybridization-based detection and analysis of mutations and polymorphisms ("genotyping"), and in a wide range of gene expression studies. The manufacturing process integrates solid-phase photochemical oligonucleotide synthesis with lithographic techniques adapted from the microelectronics industry. The present-generation methodology employs MeNPOC photo-activatable nucleoside monomers with proximity photolithography, and is currently capable of printing individual 10 mum(2) probe features at a density of 10(6) probes/cm(2).

引用

页码：525 / 531

页数：7

共 13 条

[1]

Beecher J.E., 1997, POLYM MAT SCI ENG, V77, P394

[2]

BEECHER JE, 1997, POLYM MAT SCI ENG, V76, P597

[3]

Beier M, 2000, Nucleic Acids Res, V28, pE11, DOI 10.1093/nar/28.4.e11

[4] LIGHT-DIRECTED, SPATIALLY ADDRESSABLE PARALLEL CHEMICAL SYNTHESIS [J].