High conservation of transcription factor binding and evidence for combinatorial regulation across six Drosophila species

被引:104
作者
He, Qiye [2 ]
Bardet, Anais F. [1 ]
Patton, Brianne [2 ]
Purvis, Jennifer [2 ]
Johnston, Jeff [2 ]
Paulson, Ariel [2 ]
Gogol, Madelaine [2 ]
Stark, Alexander [1 ]
Zeitlinger, Julia [2 ,3 ]
机构
[1] Res Inst Mol Pathol IMP, Vienna, Austria
[2] Stowers Inst Med Res, Kansas City, MO USA
[3] Univ Kansas, Sch Med, Dept Pathol, Kansas City, MO USA
基金
奥地利科学基金会;
关键词
NEUROGENIC GENE-EXPRESSION; EARLY MESODERM DEVELOPMENT; FUNCTIONAL ELEMENTS; HUMAN GENOME; EVOLUTION; TWIST; ENHANCERS; EMBRYO; DISCOVERY; SEQUENCES;
D O I
10.1038/ng.808
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The binding of some transcription factors has been shown to diverge substantially between closely related species. Here we show that the binding of the developmental transcription factor Twist is highly conserved across six Drosophila species, revealing strong functional constraints at its enhancers. Conserved binding correlates with sequence motifs for Twist and its partners, permitting the de novo discovery of their combinatorial binding. It also includes over 10,000 low-occupancy sites near the detection limit, which tend to mark enhancers of later developmental stages. These results suggest that developmental enhancers can be highly evolutionarily constrained, presumably because of their complex combinatorial nature.
引用
收藏
页码:414 / +
页数:8
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