Expression of a cyanobacterial Delta(6)-desaturase gene results in gamma-linolenic acid production in transgenic plants

被引:77
作者
Reddy, AS [1 ]
Thomas, TL [1 ]
机构
[1] TEXAS A&M UNIV, DEPT BIOL, COLLEGE STN, TX 77843 USA
关键词
tobacco; octadecatetraenoic acid; novel plant lipids;
D O I
10.1038/nbt0596-639
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Gamma-linolenic acid (GLA), a nutritionally important fatty acid in human and animal diets, is not produced in oil seed crops. Many oil seed plants, however, produce significant quantities of linoleic acid, a fatty acid that could be converted to GLA by the enzyme Delta(6)-desaturase if it were present. As a first step to producing GLA in oil seed crops, we have cloned a cyanobacterial Delta(6)-desaturase gene. Expression of this gene in transgenic tobacco resulted in GLA accumulation. Octadecatetraenoic acid, a highly unsaturated, industrially important fatty acid, was also found in transgenic tobacco plants expressing the cyanobacterial Delta(6)-desaturase. This is the first example of engineering the production of 'novel' polyunsaturated fatty acids in transgenic plants.
引用
收藏
页码:639 / 642
页数:4
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