Coulometric electrochemical detection of phospholipid hydroperoxides by highperformance liquid chromatography

被引：6

作者：

Arai, H

Mohri, S

Suzuki, T

Takama, K

Terao, J

机构：

[1] MINIST AGR FORESTRY & FISHERIES, NATL FOOD RES INST, TSUKUBA, IBARAKI 305, JAPAN

[2] HOKKAIDO UNIV, FAC FISHERIES, DEPT MARINE BIORESOURCES CHEM, HAKODATE, HOKKAIDO 041, JAPAN

[3] MIYAGI PREFECTURAL INST TECHNOL, SENDAI, MIYAGI 982, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 01期

关键词：

HPLC; coulometric electrochemical detection; phospholipid hydroperoxide;

D O I：

10.1271/bbb.61.191

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique for the sensitive and convenient determination of phosphatidylcholine hydroperoxides was developed that uses coulometric electrochemical detection in a high-performance liquid chromatographic analysis, The detection limit for phosphatidylcholine hydroperoxides by this method was 20 pmol at a signal-to-noise ratio of 3, The glutathione-dependent phospholipid hydroperoxide-reducing activity of fresh human plasma was ascertained by this method.

引用

页码：191 / 193

页数：3

共 19 条

[1]

AKASAKA K, 1988, ANAL LETT, V21, P965

[2] NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH A FLUOROMETRIC POSTCOLUMN DETECTION SYSTEM FOR LIPID HYDROPEROXIDES [J].