NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH A FLUOROMETRIC POSTCOLUMN DETECTION SYSTEM FOR LIPID HYDROPEROXIDES

被引：33

作者：

AKASAKA, K ^{[1
]}

OHRUI, H ^{[1
]}

MEGURO, H ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,DEPT APPL BIOL CHEM,TSUTSUMIDORI AMAMIYAMACHI 1-1,AOBA KU,SENDAI 981,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 628卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)80329-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hydroperoxides (HPO) of triacylglycerols (TG) and cholesterol esters (ChE) were selectively determined at picomole levels with a fluorescence detector by postcolumn reaction with diphenyl-1-pyrenylphosphine. Hydroperoxides were separated on a normal-phase silica gel column with gradient elution with n-hexane-1-butanol. With this system, TG-HPO and ChE-HPO were separated according to their class and determined in the range 5-1000 pmol. Detection limits of hydroperoxides of cholesterol linolate and trilinolein were about 2 pmol (signal-to-noise ratio = 3) and the relative standard deviations of their peak areas were 3.4% (39.1 pmol, n = 7) and 1.8% (32.4 pmol, n = 7), respectively.

引用

页码：31 / 35

页数：5

共 19 条

[1]

AKASAKA K, 1987, ANAL LETT, V20, P731

[2]

AKASAKA K, 1987, ANAL LETT, V20, P797

[3]

AKASAKA K, 1988, ANAL LETT, V21, P965

[4] A SIMPLE FLUOROMETRY OF HYDROPEROXIDES IN OILS AND FOODS [J].

AKASAKA, K ;

SASAKI, I ;

OHRUI, H ;

MEGURO, H .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (04) :605-607

[5]

AKASAKA K, 1992, J CHROMATOGR, V596, P197

[6] MECHANISM OF THE REARRANGEMENT OF LINOLEATE HYDROPEROXIDES [J].

CHAN, HWS ;

LEVETT, G ;

MATTHEW, JA .

CHEMISTRY AND PHYSICS OF LIPIDS, 1979, 24 (03) :245-256

[7]

CRAWFORD CG, 1984, LIPIDS, V15, P325

[8]

GLAVIND J, 1952, ACTA PATHOL MIC SC, V30, P1

[9]

HARA S, 1988, J JPN OIL CHEM SOC, V37, P541

[10]

HARA S, 1990, J JPN OIL CHEM SOC, V39, P629

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