GUS expression in blueberry (Vaccinium spp.):: factors influencing Agrobacterium-mediated gene transfer efficiency

被引：42

作者：

Cao, X ^{[1
]}

Liu, Q ^{[1
]}

Rowland, LJ ^{[1
]}

Hammerschlag, FA ^{[1
]}

机构：

[1] USDA ARS, Fruit Lab, Beltsville, MD 20705 USA

来源：

PLANT CELL REPORTS | 1998年 / 18卷 / 3-4期

关键词：

Agrobacterium tumefaciens; beta-glucuronidase; GUS expression; highbush blueberry;

D O I：

10.1007/s002990050569

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Several factors were investigated for their influence on the transfer of an intron-containing beta-glucuronidase (GUS) gene into blueberry (Vaccinium spp.) leaf explants during the early stages of Agrobacterium-mediated gene transfer, including days of cocultivation, strain of Agrobacterium tumefaciens? explant age and genotype. The number of GUS-expressing leaf zones and calli were counted immediately and 2 weeks after cocultivation, respectively, to evaluate the gene transfer process. Agrobacterium tumefaciens strain EHA105 (pEHA105/p35SGUSint) was significantly more effective for transformation than strain LBA4404 (pAL4404/p35SGUSint). Four days of co-cultivation with A. tumefaciens strain EHA105 yielded about 50-fold more GUS-expressing zones than 2 days of cocultivation. Significant differences among cultivars were observed for both GUS-expressing leaf zones and calli. For some cultivars, explant age influenced the number of GUS-expressing leaf zones and calli. In most cases, the number of GUS-expressing calli was highest in those cultivars where GUS expression in the leaves was high.

引用

页码：266 / 270

页数：5

共 24 条

[11] NEW AGROBACTERIUM HELPER PLASMIDS FOR GENE-TRANSFER TO PLANTS [J].