Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane foam as a cell culture substratum and its application to a hybrid artificial liver

被引：79

作者：

Ijima, H ^{[1
]}

Nakazawa, K ^{[1
]}

Mizumoto, H ^{[1
]}

Matsushita, T ^{[1
]}

Funatsu, K ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Chem Engn Grp, Higashi Ku, Fukuoka 81281, Japan

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 1998年 / 9卷 / 07期

关键词：

polyurethane foam; artificial liver; spheroid; mammalian cell; primary hepatocyte; packed-bed bioreactor; animal experiment;

D O I：

10.1163/156856298X00136

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Monkey kidney cells (Vero), human embryonic kidney cells (293), human liver cells (PLC/PRF/5), and primary rat, dog, and porcine hepatocytes formed spherical multicellular aggregates (spheroids) in the pores of polyurethane foam which was used as a cell culture substratum, These spheroids of various cell types express high cell activity for a long period. A practical hybrid artificial liver support system composed of a multi-capillary polyurethane foam packed-bed type cell culture module including primary hepatocyte spheroids was developed, The success of the system is indicated by an 80% recovery rate in hepatic failure rats which died in control experiments.

引用

页码：765 / 778

页数：14

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